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After identifying differentially expressed genes, it’s critical to interpret these findings in a biological context. This workshop will explore the powerful downstream analyses you can perform with differential gene expression analysis results in R. Participants will learn how to conduct Gene Set Enrichment Analysis (GSEA) to uncover pathway-level insights and build Protein-Protein Interaction (PPI) networks to identify functional connections between genes. Emphasis will be placed on the biological interpretation of results, helping participants connect RNA-seq data to larger biological systems. Through hands-on exercises, attendees will apply tools like fgsea and GOrilla, enhancing their ability to extract meaningful insights from RNA-seq experiments.